Laser assisted hatching is a method under which Zona Pellucida, a glycoprotein layer hemming in the plasma membrane of mammalian oocytes, is slightly opened through an anodyne laser to attenuate the upper layer of embryo so that it can be removed from Zona Pellucida and placed in a microscope. The Zona holds a vital role in fertilisation process as only one sperm can perforate the Zona and fuse with the egg to fructify fertilisation.
It takes less time and protects from toxic chemicals. It is useful under following procedures :-
a) Who’ve failed previous IVF/ICSI cycles
b) Who have frozen embryo transfer
c) Women who are older than 37 years of age hence produce eggs with a harder Zona Pellucida
d) Women who have a high Follicle Stimulating Hormone (FSH) level.